Phd Position To Work On A Horizon Europe Funded Project
2 semanas atrás
Organisation/Company: Nantes Université
Department: Medecine
Research Field: Biological sciences » Biology, Medical sciences » Health sciences
Researcher Profile: First Stage Researcher (R1)
Positions: PhD Positions
Country: France
Application Deadline: 31 Dec 2024 - 00:00 (Europe/Paris)
Type of Contract: Temporary
Job Status: Full-time
Offer Starting Date: 2 Sep 2024
Is the job funded through the EU Research Framework Programme? Horizon Europe (other)
Is the Job related to staff position within a Research Infrastructure? No
Offer Description To investigate the effects of pneumonia-induced immune reprogramming on the progression of atherosclerosis, we will follow up wild-type, apoE-/- and LDLR-/- mice (which spontaneously developed atherosclerosis) 16 weeks after the cure from a non-lethal model of bacterial pneumonia. We will then compare atherosclerosis lesions between infection-cured mice and mice left uninfected. Specific activities within this task include:
CVRD progression will be investigated by longitudinal measures of the blood levels of lipids (LDL, HDL, PCSK9 every week for eight weeks) and the development of atherosclerosis under an atherogenic diet by aortic isthmus histological analyses (at 16 weeks).
Immune cell compositions of atheroma lesions will be studied by single-cell RNA-sequencing of cell suspension obtained after enzymatic and mechanical digestion of aortic intima. The bioinformatics analysis will define a list of immune mediators which can mediate atherosclerosis progression.
Immune modulation to prevent atherosclerosis progression after pneumonia. Out of all the potential targets for modulation proposed above, we will prioritize those common to molecules tested in human cohorts (WP2), endotypes defined in WP4 and those with easy access to mAb and genetically modified mice. We will then functionally validate the role of these mediators by comparing the progression of atherosclerosis (same method as above) in pneumonia-cured mice treated or not with blocking antibodies for the immune targets and in mice with conditional gene deletion.
Where to apply E-mail: ******
Requirements Research Field: Medical sciences » Health sciences
Education Level: Master Degree or equivalent
Skills/Qualifications: Basic training in Immunology and animal models
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