Post-Doc (M/F) -Effect Of Host Genetics On Pathogen-Pathogen Interactions
2 semanas atrás
Organisation/Company: CNRS
Department: Laboratoire des Interactions Plantes Microbes Environnement
Research Field: Biological sciences » Botany, Biological sciences Technology » Biotechnology
Researcher Profile: First Stage Researcher (R1)
Country: France
Application Deadline: 29 Nov 2024 - 23:59 (UTC)
Type of Contract: Temporary
Job Status: Full-time
Hours Per Week: 35
Offer Starting Date: 1 Apr 2025
Is the job funded through the EU Research Framework Programme? Horizon 2020
Is the Job related to staff position within a Research Infrastructure? No
Offer Description
Infectious disease is often the major selective agent in nature, and understanding how natural populations evolve requires knowledge of their pathogenic microbes. An important factor determining the ability of pathogens to invade and proliferate in a host is the resident microbiota. We are beginning to understand its diverse impacts and interactions among pathogenic microbes. The PATHOCOM project aims to integrate large-scale field observations of microbiota/pathobiota in the plant Arabidopsis thaliana with experimental tests of host-dependent interactions among microbes.
This postdoctoral project is part of the third step of the PATHOCOM project, which aims to test the effect of the genetics of A. thaliana on pairwise interactions between 6 bacterial pathogenic strains (3 strains of Pseudomonas syringae and 3 strains of Xanthomonas campestris). The recruited post-doc will (i) set up large GWAS in in vitro and field conditions, and (ii) functionally validate several QTLs using modern plant genome editing tools (e.g. CRISPR/Cas9). The post-doc will work with a panel of 200 Illumina whole-genome sequenced A. thaliana accessions from the southwest of France, including 50 accessions sequenced with PacBio technology.
This 24-month postdoctoral project (with a possible 12-month extension) is funded by the ERC (European Research Council) and will involve collaboration with research groups at the Max Planck Institute (Tübingen, Germany) and New York University (USA).
The post-doc will be responsible for setting up GWA mapping experiments to test the effect of 200 natural accessions of A. thaliana on 6 pairs of pathogenic strains under in vitro and field conditions. While GWAS in vitro will be conducted in France (Toulouse), field conditions will be tested in the USA (3-4 stays of 6 weeks each). The post-doc will also validate several QTLs using advanced plant genome editing tools and will collaborate with another postdoc on the project with complementary skills.
Applicants should possess excellent skills in molecular biology to validate candidate genes underlying plant QTLs affecting pathogen-pathogen interactions. Mastery of the latest plant genome editing tools is essential. Skills in quantitative genetics, experimental design, and statistical analysis are advantageous. Fluent English is required.
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