Biochemist Researcher Wanted for Advanced Bacterial Secretion System Studies
Há 6 dias
Cnrs is seeking a skilled biochemist to join our research team at the CBMN laboratory (UMR5248). The successful candidate will be responsible for investigating the molecular mechanisms of bacterial secretion systems under control of the second messenger c-di-GMP.
Bacterial secretion systems are membrane macrocomplexes that play a vital role in biofilm formation, bacterial cell physiology, and pathogenicity. They are also crucial targets in the development of new anti-infective drugs.
This project aims to understand the function and pharmacology of these macromolecular systems.
The position is assigned to the CBMN laboratory within the team of Dr. Petya Krasteva, who specializes in "Structural Biology of Biofilms."
In this role, you will develop protocols for expressing and purifying proteins and membrane, cytosolic, and periplasmic complexes in various heterologous systems. You will also conduct solubilization and protein stability tests, as well as functional assays.
You will perform protein crystallization screens and contribute to designing crystal optimization methods. Additionally, you will collect data at synchrotron facilities in France and abroad using X-ray diffraction and state-of-the-art cryo-electron microscopy (cryoEM).
Your responsibilities will include preparing samples for cryo-EM, collecting and analyzing data, and communicating your findings through presentations and scientific publications.
The offer includes a 15-month contract funded by an ANR project, with the possibility of extension based on performance.
Key Responsibilities:- Develop protocols for expressing and purifying proteins and complexes
- Conduct solubilization and protein stability tests, as well as functional assays
- Perform protein crystallization screens and contribute to designing crystal optimization methods
- Collect data at synchrotron facilities using X-ray diffraction and cryoEM
- Analyze data and communicate findings through presentations and publications
We are offering a competitive salary of approximately €35,000 - €45,000 per year, depending on experience, plus additional benefits.
We encourage researchers from under-represented groups to apply.
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